Journal: Biochemistry and Biophysics Reports
Article Title: Integrated multi-omics analysis reveals TMEM147 as an immunosuppressive prognostic biomarker in LUAD
doi: 10.1016/j.bbrep.2025.102281
Figure Lengend Snippet: TMEM147 promotes the proliferation, migration and invasion of LUAD in vitro (A) The protein expression levels of TMEM147 in BEAS2B, HCC827, NCI–H1975, NCI–H1299, A549 and PC9 cell lines were detected. (B) Western blot analysis of TMEM147 expression in NCI–H1299 and A549 cells (siTMEM147-1,siTMEM147-2 and control). (C–D) NCI–H1299 and A549 cells were used to determine clone formation assays. (E–G) Transwell assays showing migration and invasion abilities. (H–I) Wound-healing assays. Scale bars: Scale bars: 100 μm (100 × ), 50 μm (200 × ).∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, no significance.
Article Snippet: The human lung epithelial cell line BEAS2B and four LUAD-derived cell lines (A549, NCI–H1299, HCC827 and NCI–H1975) were acquired from ATCC.
Techniques: Migration, In Vitro, Expressing, Western Blot, Control